Contact an Account ManagerDetermination of L-dopa and 3-O-methyldopa in human plasma by extraction using C18 cartridges followed by HPLC analysis with electrochemical detection
Summary
Type: HPLC
Application #: 105190
Catalog#: 720041.46 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4.6x250mm
Detection: electrochemical, glassy carbon electrode, 0.75V versus Ag/AgCl
Conditions: eluent: 0.1M NaH2PO4 - methanol (84:16), containing 2.6mM Na octyl sulphonate, 0.1mM Na2EDTA and 0.25mM triethylamine, pH adjusted to 3.35 with orthophosphoric acid
flow rate 1.5ml/min
Authors: Gerlach M
Source: J Chromatogr 380 (1986) 379 - 385
Details
Legend: Fig. 1. HPLC chromatograms of A) 100µl standard mixture of 750ng/ml L-dopa and
2000ng/ml 3-OMD in HPLC buffer, B) 100µl ethanolic eluate from the plasma of a
healthy male proband treated with 50mg o